Solution Informationhelp
Enzyme: Tyrosine-protein phosphatase non-receptor type 7
inhibitor: BDBM31757
substrate: n/a
 
Solution Type: Aqueous
pH at Preparation: n/a
Temp. Prep.: n/a
Comments: HePTP assay materials: 1) HePTP protein was provided by Dr. Mustelin (Sanford-Burnham Medical Research Institute, San Diego, CA). The pNPP pellets were obtained from Sigma-Aldrich (S0942). Biomol Green reagent was purchased from BIOMOL Research Laboratories, Inc (AK-111) 2) Assay Buffer: 50 mM Bis-Tris, pH 6.0, 2.5 mM DTT, 0.0125% Tween 20. 3) HePTP working solution contained 6.875 nM HePTP in assay buffer. Solution was prepared fresh prior to use. 4) pNPP working solution contained 1 mM pNPP in MQ water. Solution was prepared fresh prior to use. 5) Vanadate working solution - 45 mM Na3VO4 in 10% DMSO HePTP HTS protocol: 1) 4 uL of 100 uM compounds in 10% DMSO were dispensed in columns 3-24 of Greiner 384-well clear microtiter plates (781101). 2) 4 uL of the following solutions were using the Multidrop bulk dispenser (Thermo): a. 10% DMSO column 1 (negative control) b. 45 mM Na3VO4 in 10% DMSO - column 2 (positive control). 3) 8 uL of HePTP working solution was added to the whole p
 
 
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